human-murine fgf8  (PeproTech)

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: A-F, mafB expression at various HH stages indicated in the boxes above the panel. G-L FGF8 expression at the HH stages indicated in the boxes. M, N FGF8 and BrdU expression at HHst30 following BrdU application at st 16 (M) or st 19 (N) O FGF8 and BrdU expression at HH st 35 following BrdU application at HH stage 18.

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: Expressing

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: FGF8 in situ hybridisation at HH stage 36 following BrdU application to embryos at HH stages 18 to 22, as indicated by the boxes above the panels A series of sections from individual embryos is shown from rostral to caudal (top panels to bottom panels) as indicated by the schema on the left hand side

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: In Situ, Hybridization

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: FGF8 expression at HH stage 39 across the entire auditory hindbrain sections are numbered sequentially from rostral to caudal most and are indicated in the panels

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: Expressing

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: FGF8 expression at HH stage 30 across the entire auditory hindbrain sections are numbered sequentially from rostral to caudal most and are indicated in the panels

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: Expressing

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: FGF8 expression at HH stage 33 across the entire auditory hindbrain sections are numbered sequentially from rostral to caudal most and are indicated in the panels

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: Expressing

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: FGF8 expression at HH stage 36 across the entire auditory hindbrain sections are numbered sequentially from rostral to caudal most and are indicated in the panels

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: Expressing

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: A-D FGF8 expression at HH stage 30-39 indicated in the boxes above the panels. E-G FGF8 expression at HH stage 33 annotated to indicate the medial (Wm) Central (Wc) and Lateral (Wl) widths of the nL and the Lengths (L) of the nucleus. H schematic of that shown in E-G. I Graphs showing length to width ratios of the nL in Medial, Central and Lateral regions at HH stages 30, 33 and 36. J-L FGF8 expression at HH st 33 (J,L) related to Caspase-3 expression in the nL compared to a control area of the hindbrain (K) M potential models of lamina formation of the nL by spatially restricted cell death or cell movement.

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: Expressing, Control

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: A-I FGF8 (A,D,G), cadherin-22 (B, E, H) and cadherin-13 C, F, I) expression in the nM, nL and nA at HH stage 39, 32 and 30, as indicated in the left hand boxes. J-O Cadherin-2 ( N-cadherin ) expression in the hindbrain at rhombomere 5/6 at HH stages 17 (J) 21 (K) 23 (L) 26 (M) 30 (N) and 35 (O) P-R. ψ-catenin (P) and cadherin-2 (Q) expression in the nM and nL related to DAPI expression (R) at HH stage 36.

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: Expressing

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: ψ-catenin, N-cadherin, N-cadherin and FGF8 expression at HH stage 36 top panels Various sections across the auditory hindbrain are shown. Bottom panels show a time series of expression in the auditory hindbrain at HH stage 30, stage 33 and stage 34

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: Expressing

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: A-C FGFR1 (A) FGFR2 (B) and Sprouty-4 (C) expression at HH stage 34 D-F control in ovo electroporation of GFP at HH st 18 followed by expression of GFP (D) FGF8 (E) and cadherin-22 (F) at HH stage 30. G-I mFGF8-GFP in ovo co-electroporation at HH st 18 followed by GFP (G) and islet-1 (H) expression at rhombomere 8 at HH stage 30. I Quantitation of motor neuron number at r8 of mFGF8 electroporation compared to control. J-M FGF8 (J,L) and cadherin-22 expression (K,M) following mFGF8-GFP co-electroporation or on the control side of the hindbrain as indicated in the left-hand boxes. N Quantitation of FGF8 or cadherin-22 cells in the nL area at HH st 34 following mFGF expression compared to control. O Quantitation of FGF8 or cadherin-22 cells in the nL area at HH st34 following dn FGFR1 expression compared to control. P-R dnFGFR-1-GFP co-electroporation at HH st18 followed by analysis of GFP (P), FGF8 (Q) and cadherin-22 (R) expression at HH stage 34.

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: Expressing, Control, In Ovo, Electroporation, Quantitation Assay

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: A-D r 2 measures of lamina formation for each section of FGF8 in situ expression at HH stage 30 (A), st33 (B) st 36 (C) and st 39 (D) see materials and methods for details. E-L. FGF8 (E-H) and MafB (I-L) expression in hindbrain slices cultured in varying concentrations of exogenously added FGF8 indicated in the boxes above). M, N Box and whisker plots of r 2 calculations of wildtype at different HH stages and in different concentrations of FGF8 in culture conditions (N)

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: In Situ, Expressing, Cell Culture, Whisker Assay

Journal: bioRxiv

Article Title: FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

doi: 10.1101/2025.04.30.651488

Figure Lengend Snippet: A-F In ovo electroporation at stage 19 of either N-cadherin (A, B) or dominant negative N-cadherin (C-F) doxicycline was added at HH stage 30 and analysis was at HH stage 36. GFP (A, C) marks electroporated cells and axons. Analysis of nL/nM is shown by FGF8 expression (B, E), cadherin-22 (D) or cadherin-13 expression (F). Experimental or control sides of the hindbrain are shown as indicated in the boxes above.

Article Snippet: Electroporated slices are immediately put in chicken 1-2ml of culture media fortified with varying concentrations of FGF8 (Human-murine FGF8, CN:100-25, Peprotech) and placed in sterile 5% CO2 incubator and cultured at 37°C.

Techniques: In Ovo, Electroporation, Dominant Negative Mutation, Expressing, Control